by Donald Voet & Judith Voet
Chapters 4-9, 29
3 semester hours. The goal of the course is to provide a critical understanding of the relationship between structure and function of biological macromolecules such as proteins and nucleic acids. The laboratory provides hands-on experience with state-of-the-art equipment used in the biotech industry. Methods presented will include PCR, Plasmid purification, Gel electrophoresis (Agarose, SDS,2D), Restriction Digestion, Sequencing, Denaturing Gradient Gel Electrophoresis, Bradford, ELISA, Chromatography and etc. The laboratory experiments will allow students to gain experience in using computer applications related to the determination of the structures and properties of biological molecules and recent bioinformatics tools. The class meets for 2 hours of lecture each week. An additional 4-8 hours per week is spent learning the laboratory techniques.
Add/Drop Policy. Students can add up until the 4th class day. Movement into and out of classes after the 4th class day requires approval of the Department Chair. Students can drop until mid-semester without a WP or WF. Drops after mid-semester require approval of the Dean. You are responsible for your enrollment status with the college.
Regular or punctual attendance is expected but in this class attendance is not taken at any class or laboratory meeting. You are responsible for obtaining any information distributed during those times.
Academic dishonesty will not be tolerated. Dishonesty will result in assignment of a failing grade on that exercise or examination.
(Plagiarism is the act of obtaining or attempting to obtain credit for academic work by representing the work of another as one's own without the necessary and appropriate acknowledgment.)
Cheating is the act of obtaining or attempting to obtain credit for academic work by using dishonest means: submitting as one's own work an examination, paper, research which has been purchased, borrowed, or stolen; or fabricating data; or using of electronic devices, not specifically authorized by the instructor during an examination)
Excused absences
Please consult the SFASU General Bulletin for the excused absence policy. Laboratory Schedule
The class will meet in the Distance Learning class for a short pre-lab (introduction). You are expected to complete the hands-on laboratory work during the scheduled meeting times.
Laboratory notebook. Roaring Spring Cat# 77649, quad-ruled with duplicate pages. Available at the University Bookstore.
| Biochemistry (3rd Edition) by Donald Voet & Judith Voet Chapters 4-9, 29 | |
| DNA Structure and Function by Richard R. Sinden |
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| Introduction to Protein Structure by Branden C. & Tooze J. |
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| Introduction to Protein Architecture: The Structural Biology of Proteins by Arthur M. Lesk |
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Essentials of Writing Biomedical Research Papers 2nd edition by Mimi Zeiger
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Laboratory Notebook/Performance
10%
Midterm examinations
2X20%
Lab quizzes
10%
Lecture quizzes
10%
Laboratory Reports
30%
Total
100%
89 – 100% =A 79 – 88% =B
69 – 78% =C
59 – 68% =D
Notebooks will be signed after every laboratory by teaching assistants. Notebook should be hardbound. Notebook must represent real-time data, methods and results. There should be scratch-outs, scribbles, notes, drawings, calculations, etc. Notebook must include: date, title of experiment, purpose of experiment and original data. Notebook should include vendor and lot numbers of reagents used and the name and model numbers of the equipment used. Notebook must contain original figures and data. Performance will be based on your punctuality, preparation, time management and participation. Learn more follow this link How to Develop a Laboratory Notebook
See the schedule of topics for the due dates. Must be typed (double-spaced). Include near publication quality figures, tables.
Use publication format, include:
See the schedule for dates and times. Format will include but is not limited to multiple choice, short answer and essay type questions. Context will include any material covered during lecture or laboratory.
Before each lab there will be a short quiz which will cover the material required to be studied before the lab (lab protocols and required reading will be available on the web). Lab quizzes will be a combination of multiple choice, matching, short answer, and practical questions. If you miss a lab quiz, you will not be allowed to make it up unless you have a valid excuse.
Before each lecture there will be a short quiz which will cover the material learned since the previous quiz (required and recommended reading: chapters and articles will be available on the web). Lecture quizzes will be a combination of multiple choice, matching, short answer, and practical questions. If you miss a lecture quiz, you will not be allowed to make it up unless you have a valid excuse.